品牌：Mirus

貨號：MIR 7021

品名：Label IT® Tracker™ Intracellular Nucleic Acid Localization Kits

產(chǎn)品信息：

產(chǎn)品類型：核酸定位試劑盒

規(guī)格：每個試劑盒包含Label IT®Tracker™試劑，Label IT®重組液，和10X標記緩沖液。

儲存條件：-20℃

保質(zhì)期：1年（重組后6個月）。

產(chǎn)品介紹：

特點：

多功能標簽-高效標簽和可視化的DNA。

一步化學法-易于和一致地控制標記反應。

高效標記-細胞中DNA的*佳可視化。

卓/越追蹤-在轉(zhuǎn)染標記(質(zhì)粒)DNA后監(jiān)測亞細胞定位和報告轉(zhuǎn)基因表達。

相關(guān)數(shù)據(jù)：

Label IT® Tracker™ Kits. The Label IT® Tracker™ labeling reagents are composed of three regions: the label (a fluorophore or biotin) (green), the linker (yellow) which facilitates electrostatic interactions with plasmid DNA and the reactive alkylating group (blue) that covalently attaches the Label IT® reagents to any reactive heteroatom within the plasmid. Attachment of the Label IT® Reagent to plasmids does not alter their structure or affect downstream gene expression, and as such, the same labeled plasmid can be both visualized after transfection by fluorescence microscopy and employed to express the gene of interest on the plasmid.

Tracking of Plasmid Localization and Expression. COS-7 cells transfected with Label IT® Tracker™ Cy®5 (blue) labeled pEYFP-nuc and TransIT®-LT1 Reagent in serum containing media. Images were acquired at 3, 8, and 20 hours post-transfection. The blue signal indicates the cellular localization of the labeled plasmid, the green signal indicates cellular autofluorescence, and the yellow signal is the expression of the nuclear yellow fluorescent protein (YFP) reporter.

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